The Systems Biology module is a major redesign of the former Flow Cytometry module. The Flow Cytometry module has existed in our Core Grant for 10 years and remains heavily utilized by a significant portion of our faculty, so we will maintain this function. However, the development of -omics and cell-based analysis has dramatically increased our need to reinvent this module as a Systems Biology module. The module has been redesigned to combine flow cytometry with flow sorting for enrichment of phenotypic cell populations for downstream multi-omic RNA, chromatin, protein, or metabolic analysis. Sorted cells can be used for bulk RNA-Seq, and chromatin analysis by ATAC-Seq, ChIP-Seq, and cut-and-run. In addition, the module will be enhanced to include single-cell RNA-Seq/ATAC-Seq and single-nuclei RNA-Seq/ATAC-Seq.