For more information on the Systems Biology module or to request services, please contact Anthony St. Leger at Anthony.stleger@pitt.edu
The Systems Biology Core at the Department of Ophthalmology, University of Pittsburgh, is a cutting-edge facility focused on FACS-based cell analysis and sorting. We also help support single-cell analysis of bulk and sorted populations of cells from various species to include mouse, zebrafish, nonhuman primate, human, and others. Our hallmark cell analyzers are the Beckman Coulter Cytoflex LX and Sony ID7000, both pieces of equipment are powerful tools for phenotypic characterization and can analyze up to 14 and 55 parameters, respectively. The Sony MA900 and MACS Tyto provide complementary cell sorting capabilities, with the former sorting up to four populations at low pressure and the latter using a gravity-based approach for low-pressure sorts and maximum cell viability. These instruments support a range of applications, from detailed cell profiling to the isolation of cells for advanced molecular analyses like RNAseq and chromatin studies.
Downstream of cell sorting, the core is equipped with instruments that can assist users in the preparation of single-cell RNAseq/ATACseq libraries and multiome library prep. Those instruments include a cell counter, tapestation, and 10X Chromium machine.
Backed by daily quality control and expert staff support, the core is a vital resource for advancing ophthalmology research at the University of Pittsburgh.